Relationship of the Genomic Complexity of Hepatitis C Virus With Liver Disease
Severity and Response to Interferon in Patients With Chronic HCV Genotype 1b
Infection
Fig. 1. Estimation of the sensitivity of the PCR-SSCP
assay. Lane 1, molecular weight marker. Reciprocal amounts of cDNA from
two isolates (A and B) producing a different SSCP pattern were mixed, amplified
by PCR, and submitted to SSCP analysis (lanes 2 to 14). Bands in
the upper and lower parts of the gel correspond to HCV variants present in isolate
A and in isolate B, respectively. Variants representing at least 5% of the total
viral population present in the mixtures produced visible bands in the gel (arrows).
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Copyright © 1999 by the American Association for the Study of Liver Diseases.
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