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Molecular Basis for Persistent Hepatitis B Virus Infection In the Liver
After Clearance of Serum Hepatitis B Surface Antigen
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table 1. Southern Blot Studies of Hepatic
Nucleic Acids Amplified by Either RT-PCR Using Primers Complementary
to the HBV Core and Surface Genes or Seminested PCR Using Primers
Flanking the HBV DR Region
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|
| Patients |
Months After HBsAg Clearance |
RT-PCR for HBV RNA
|
Seminested PCR
|
| HBV Core Gene Primers |
HBV Surface Gene Primers |
HBV DR Region Primers |
|
| Study group* |
| 1 |
3 |
+ |
+ |
+ |
| 2 |
3 |
+ |
+ |
.gif) |
| 3 |
9 |
|
+ |
+ |
| 4* |
24 |
|
|
|
| 5 |
46 |
|
|
+ |
| 6 |
47 |
|
|
|
7 |
67 |
+ |
+ |
|
| Controls |
| HBsAg-positive |
|
12/13 |
11/13 |
3/3 |
| HBsAg-negative |
|
0/4 |
0/4 |
0/4 |
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* All study group
patients HBV-DNA-positive in the liver by PCR except for patient 4.8
Patients 1, 2, and 3 represent patients 1, 2, and
3 in Kuhns,8 whereas patients 4, 5, 6, and 7 in
this table are patients 7, 4, 5, and 6, respectively,
as tabulated in Kuhns.8
HBV-DNA-positive in serum by PCR.8 |
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