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Evaluation of Assays for Antibody to Hepatitis E Virus by a Serum Panel

table  3.   Antibody to HEV Endpoint Dilution by 12 Different Tests in Early-Convalescent Human Sera Diluted With Normal Human Serum
Dilution Test
1 2 3 4 5 6 7 8 9 10 11 12
NHS  -  -  -/dagger  -  -  -  -  -  -/dagger  -  -  -
1:0 + + + + + + + + + + + +
1:5 + + + + + + + + + Dagger + +
1:10 + + + + + + + +  -  - +/- +
1:20 + + + Dagger + + + + +  -  - +/-
1:40 + +  - +/-  - + +  - + -  -  -
1:80 + +  -  -  -  -/dagger +  -  -  - - -  -
1:160  - +  -/ dagger  -  -  - +  - +/-  -  -  -
1:320  -  -  -/ dagger  -  -  -  -  - +  -  -  -
1:640  -  - -/dagger  -  -  -  -  - +  -  -  -
1:1280  -  - -/dagger  -  -  -  -  - +/dagger  -  - -
* Two aliquots of each dilution were tested; +, both aliquots were reactive; +/-, one aliquot was reactive and one was negative; -, both aliquots were negative.
 dagger / One aliquot had an indeterminate result (discordant results by enzyme immunoassay or weakly reactive by Western blot).
Dagger Two aliquots had an indeterminate result.

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