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Hepatitis B DNA General Clinical Tests

Test Name
Method
Hepatitis B DNA (HBV DNA)

Slot bar or liquid hybridization

Specimen
Requirements
Serum
Freeze if test can not be run immediately. Stable for at least 7 d at -20°
Ref. Range
Conventional
International Recommended Units
Negative
(Limits of detection: -3 x 10 (4) genome eqivalents/mL)
Chemical Interfaces
In Vivo Effects
None Found
Diagnostic Information The presence of HBV-DNA in serum confirms active Hepatitis B infection and implies infectivity of serum. Current use of the assay is for confirming Hepatitis B infection in immunosuppressed patients with confusing serological markers and in assessing responses of Hepatitis B to therapy, such as intron -a.
Remarks Hepatitis B DNA and RNA can also be detected in tissues using molecular biological techniques, but these tests are generally available. Polymerase chain reaction (PCR) assays for detecting and subtyping Hepatitis B variants may become commercially available within the next few years.

References:
Clinical Guide to Laboratory Tests, third edition.

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