Effects of Chronic Ethanol Administration on Rat Liver
Proteasome Activities: Relationship With Oxidative
Stress
Fig. 2. SDS-polyacrylamide gel
electrophoresis of proteasome and peptidase activities. (A) Western
blotting was performed using rabbit anti-rat proteasome antiserum
as described in Materials and Methods. Lanes 1-2: purified
rat liver proteasome (0.5 and 0.25 µg,
respectively). Lane 3: 75 µg of proteins from
supernatant obtained after the centrifugation of liver homogenate
from control rats at 100,000g for 6 hours. Lane
4: 75 µg of proteins from supernatant obtained after
the centrifugation of liver homogenate from control rats at
100,000g for 1 hour. A molecular-weight marker
(33 kd) is indicated on the right side. (B) Peptidase
activities were determined using fluorogenic peptides as described
in Materials and Methods, in the same supernatants used in Western
blotting, lanes 3-4.
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